- Marine Science and Technology Bulletin
- Vol: 9 Issue: 2
- Purification of Glucose 6-Phosphate Dehydrogenase from Capoeta umbla Gill and Liver Tissues and Inhi...
Purification of Glucose 6-Phosphate Dehydrogenase from Capoeta umbla Gill and Liver Tissues and Inhibition Effects of Some Metal Ions on Enzyme Activity
Authors : Muammer Kirici, Muhammed Atamanalp, Mahinur Kirici, Şükrü Beydemir
Pages : 92-101
Doi:10.33714/masteb.709377
View : 25 | Download : 11
Publication Date : 2020-12-31
Article Type : Research
Abstract :In this study, the in vitro effects of some metal ions (Ag + , Cd 2+ , Cu 2+ , Fe 2+ , Ni 2 + and Pb 2+ ) on freshwater fish Capoeta umbla liver and gill glucose 6-phosphate dehydrogenase (G6PD) have been investigated. For this purpose, C. umbla liver and gill G6PD enzymes were purified, with a specific activity of 31.52 and 22.83 EU/mg protein, 3353.2 and 736.5 fold in a yield of 19.28% and 23.15%, respectively. In order to control the enzyme purification SDS-PAGE showed a single band for the enzyme. In addition, in vitro effects of metal ions on enzyme activity were researched. As a result, Ag + , Cd 2+ , Cu 2+ , Ni 2+ and Pb 2+ inhibited fish liver G6PD; Ag + , Cd 2+ , Fe 2+ , Ni 2 + and Pb 2 + inhibited fish gill G6PD. Besides, it was found that the most effective inhibitor of G6PD enzyme within metal ions used was Ag + . Our results also demonstrate that these metals might be dangerous at low micromolar concentrations for fish G6PD enzyme.Keywords : Glucose 6-phosphate dehydrogenase, Capoeta umbla, Enzyme purification, Inhibition, Metal ions, Liver