- Experimed
- Vol: 12 Issue: 2
- Cross-interactions between Norepinephrine, Methicillin-Resistant Staphylococcus aureus and Human Ost...
Cross-interactions between Norepinephrine, Methicillin-Resistant Staphylococcus aureus and Human Osteoblast Cells in Culture Conditions
Authors : Fatma Kalayci Yüksek, Defne Gümüş, Gül Ipek Gündoğan, Aysun Uyanik Öcal, Nur Elagül, Mine Anğ Küçüker
Pages : 85-93
Doi:10.26650/experimed.1124000
View : 22 | Download : 6
Publication Date : 2022-08-22
Article Type : Research
Abstract :Objective: The role of norepinephrine (NE) on growth, adhesion and invasion of methicillin-resistant Staphylococcus aureus (MRSA) ATTC 43300 was examined in human osteoblast (HOB) cells. The effects of NE and/or MRSA on the viability and cell death pathways of HOB cells were also investigated. Furthermore, the alterations of bacterial response to oxidative stress (H2O2) were analyzed in the presence/absence of NE. Materials and Methods: Bacterial growth was detected spectrophotometrically. The colony counting method was examined for adhesion-invasion. The alteration of HOB cell viability was determined by methyl thiazolyl diphenyl-tetrazolium bromide (MTT) assay. The death pathways of HOB cells were examined microscopically using acridine orange-ethidium bromide dual staining and dichlorofluorescein-diacetate (DCF-DA) dye. The bacterial response to H2O2 was investigated by agar dilution. Results: The growth of bacterium was not affected in the presence of NE. Bacterial adhesion was decreased by NE (p<0.0001) while high-level NE induced invasion (p=0.013). HOB cell viability was reduced by MRSA and/or NE (p<0.001). MRSA and co-existence of MRSA and NE caused necrosis more than apoptosis in HOB cells (p<0.05). NE did not alter the bacterial response to oxidative stress. Conclusion: Norepinephrine has different effects on the biological properties of both MRSA and HOB cells.Keywords : Norepinephrine, MRSA, growth, adhesion-invasion, oxidative stress response, HOB cell viability, apoptosis-necrosis