- The Eurasia Proceedings of Science Technology Engineering and Mathematics
- Issue: 3
- Isolation and Classification of Lipoxygenase from the Rind of Pistacio Khinjuke and Investigation of...
Isolation and Classification of Lipoxygenase from the Rind of Pistacio Khinjuke and Investigation of its Affinity Toward Certain Inhibitors in Mice: in vivo and in vitro
Authors : Ameera A. Al-juraisi, Omar Y. Al-abbasy
Pages : 72-84
View : 18 | Download : 5
Publication Date : 2018-12-01
Article Type : Research
Abstract :This study includes purification of lipoxygenase from the rind of pistacio khinjuke by applying ammonium sulfate precipitation (70%), dialysis, ion exchange chromatography, and the slab electrophoresis technique. One isoenzyme has been obtained with a special activity of 1230x10-4 unit/mg protein and a molecular weight of 101997 dalton. The partially purified rind lipoxygenase has been characterized and inhibited by quercitine rutinoside and melatonine. The type of inhibition seemed to be uncompetitive and the Ki values were 1.77 and 1.62 mM, respectively. Due to the importance of lipoxygenase for the animal, this study also deals with the determination of its affinity toward quercitine rutinoside and melatonine in the serum of normal and diabetic male mice. The best doses for lipoxygenase inhibition were 523.3 and 8 Mg/Kg body weight of quercitine rutinoside and melatonine, respectively. Treatment of animals with these doses led to a significant decrease in lipoxygenase activity, glucose level, and total cholesterol/high density lipoprotein cholesterol ratio in animal serum. Therefore, these natural inhibitors seem to serve as a drug for reducing the complications of some diseases (e.g. diabetes mellitus) which are affected by this enzyme.Keywords : Lipoxygenase, Melatonine, Quercitine rutinoside, Pistachio khinjuke