- The Eurasia Proceedings of Science Technology Engineering and Mathematics
- Issue: 3
- Study of Paraoxonase1 Enzyme for Women with Breast Cancer (Biochemical and Molecular Genetics Study)
Study of Paraoxonase1 Enzyme for Women with Breast Cancer (Biochemical and Molecular Genetics Study)
Authors : Luma Abdalmunim Baker, Hazha Jamal Hidayet, Nahida S. Al-chalabi
Pages : 56-71
View : 20 | Download : 6
Publication Date : 2018-12-01
Article Type : Research
Abstract :The research include studying biochemical and genetic molecular study for paraoxonase1 enzyme and identify some biochemical parameters for serum blood samples 68 women with breast cancer and follow-up after take them three doses of chemotherapy and comparative with 50 normal female as control group.The result showed for women with breast cancer a significant increase in (TC,MDA ,Total protein, U,A , calcium and BMI) when compared with normal women, the results also showed for women who have taken chemical doses and women with breast cancer significant decreased in (PON1, GSH) compared with normal women, the results showed for women who have taken the first dose significant increase in (MDA,TP, Calcium, U.A , BMI), while second dose and the third dose chemotherapy found significant increase in (TP, U.A ,Calcium ) and significant decreased for (PON1). The results included sequential analysis for PON1 enzyme ,it has shown results for genetic analyzed selected exon (Exon4), where DNA extraction for normal women and breast cancer women and taken their doses of chemotherapy and measure DNA concentration and purity using Nano-Drop Spectrophotometric Analysis where the result show the DNA concentration ranged between (5.2-68.4) ng/ml where concentration mean for women with breast cancer (26.97) ng/ml and (17.49) ng/ml for normal women while the first dose 24.74 ng/ml , (16.16-9.70)ng/ml for second and third dose respectively . It has also been used PCR technique amplification and test products PCR analysis of sequential technique by using gel electrophoresis where packet appeared clear without deformation and tested PCR product sequencing for exon4 by capillary automated sequencer and comparing the result with the normal sequence of the oxon., the results shows for women have taken first dose deletion mutation called (Remove) frame shift mutation clear nitrogen base one lead to change all codons after deletion site so that all amino acid change in poly protien chain which led to the loss of amino acid (Tyr.) . and mutation type of silent mutation third mutation type of substitution mutation (Replace amino acid (Thr.) by amino acid (Ser.).Keywords : Paraoxonase1, PON1, Chemotherapy, Molecular genetics