CONTROLLING CELL MORPHOLOGY ON ION BEAM TEXTURED POLYMERIC SURFACES
Authors : Emel Sokullu, Fulya Ersoy, Ahmet Öztarhan, Ian G. Brown
Pages : 135-141
View : 11 | Download : 4
Publication Date : 2016-02-01
Article Type : Research
Abstract :Objectives: Nano and micro sized textures on surfaces are generally useful biomaterials. Although the importance of roughness degree has been reported for cell morphology, texture morphology can be more determining for cell adhesion and proliferation. Biodegradable polymers are extensively used as scaffolds as well as implant materials in the human body. The aim of this study was to investigate the effect of ion beam bombardment on cell morphology. Methods: Gold and carbon ion implantation was made on biodegradable polymers polyglycolic acid, polycaprolactone and polylactic-co-glycolic-acid. Roughness data were obtained from atomic force microscopy. After surface modification with ion beam, B35 neural cells were evaluated on surfaces for cell compatibility. Morphology and cell surface interaction were analyzed with scanning electron microscopy. Results: We observed improved cell adhesion after ion beam surface modification. Cell adhesion was greater on gold implanted surfaces compared to the carbon implanted. Neural cells attached to lamellar wrinkles, spread by taking the shape of the pattern and exhibited high aspect ratios and axon extension. In contrast, cells that attached on the untreated surfaces remained rounded with low spreading. Conclusion: The findings of this study are important for development of ion beam modified cell cultures and scaffold systems to understand texture-based cell adhesion mechanisms.Keywords : ion implantation, cell culture, polylactic-co-glycolic acid, surface treatment, topography, wettability