- Turkish Journal of Zoology
- Vol: 32 Issue: 4
- Effects of a Neem Sample on Protein Patterns of Bactrocera cucurbitae
Effects of a Neem Sample on Protein Patterns of Bactrocera cucurbitae
Authors : Nikhat Yasmin, M. Farhanullah Khan, Muhammad Samiullah Channa, Zeeshan Ali
Pages : 415-419
View : 6 | Download : 2
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :Bactrocera cucurbitae (Coquillett) adults were reared with 5% honey solution, sugar, and protein-hydrolysate. Females were provided to lay eggs on larval rearing media in a bottle gourd. Twenty third-instar larvae of the same size and age were exposed to filter papers treated with varying doses of a neem sample in 90 mm diameter petri dishes for 24 h. A probit-mortality curve was drawn to determine the LC50 of the sample. LC50 dose of the sample was calculated as 5.6%. Thin layer chromatography was used to determine the effects of neem sample on the protein patterns of the treated insect. The Rf-values of separated proteins (peptides) were determined and compared to those of the untreated control.Keywords : Bactrocera cucurbitae, insect rearing, protein patterns, Azadirachta indica