- Turkish Journal of Veterinary and Animal Sciences
- Vol: 44 Issue: 1
- Oocyte recovery and in vitro embryo production in cows treated with a single dose of follicle-stimul...
Oocyte recovery and in vitro embryo production in cows treated with a single dose of follicle-stimulating hormone
Authors : Thiago Marquez Fernandes, Paulo Roberto Adona, Samuel Guemra, Tiago Henrique Camara De Bem, Moysés Dos Santos Miranda
Pages : 151-155
View : 10 | Download : 5
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :Cows were treated with follicle-stimulating hormone (FSH) in a single dose and then subjected to ovum pick-up (OPU) for evaluation of oocyte recovery and in vitro fertilization (IVF). Cows underwent OPU followed by application of FSH (200 mg) on day 2 and new OPU on day 3. This methodology was repeated 3 times sequentially. In FSH-free the cows (control), OPU was performed at intervals of one week. The number of oocytes recovered was higher in the control cows (22.2 ± 1.5) compared to the treated cows (16.9 ± 1.4; P 0.05). There was no difference between the control cows (47.1 ± 4.2%) and the treated cows (45.3 ± 7.7%) with respect to the percentage of embryos (P > 0.05). There were no differences in pregnancy for the blastocysts between the control and the treated cows (50.0 ± 2.1 and 47.3 ± 2.5%; P > 0.05), respectively. In conclusion, the single-dose FSH protocol did not significantly alter the evaluated results of viable oocytes, in vitro production of embryos, or pregnancy percentage. However, there is a possibility of several consecutive OPU/IVF sessions every three days.Keywords : Bovine, cryopreservation, ovum pick-up, pregnancy