- Turkish Journal of Veterinary and Animal Sciences
- Vol: 38 Issue: 5
- Impact of the depletion of high-abundance proteins in blood plasma/serum on the proteome of these me...
Impact of the depletion of high-abundance proteins in blood plasma/serum on the proteome of these media in growing farm animals
Authors : Adam Lepczynski, Alicja Dratwa-chalupnik, Agnieszka Herosimczyk
Pages : 490-495
Doi:10.3906/vet-1404-80
View : 5 | Download : 1
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :The aim of the study was to use hexapeptide combinatorial libraries to evaluate the influence of high-abundance protein depletion on low-abundance protein enrichment, protein concentration, and 2D gel quality in plasma and serum proteomes of growing piglets and calves. Depleted and nondepleted samples of plasma/serum were resolved using 2D electrophoresis (2DE). Bioinformatic analysis of the 2D gels showed an increased number of detected spots on each gel after depletion of excess high-abundance proteins in calf plasma (+26.46%) and in piglet serum (+49.40%). Relative expression of selected low-abundance protein spots in the blood plasma/serum samples was higher after high-abundance protein depletion. Both media showed decreased expressions of high-abundance protein spot clusters on 2D gels after treatment. The results of this study confirm both the high efficiency of high-abundance protein depletion and the increase in concentrations of low-abundance proteins on 2D gels after treatment. The method of depletion is reproducible and it may be used as an alternative to immunodepletion for preparing blood plasma/serum samples from young, growing animals for 2DE. Increased content of the low-abundance proteins on the 2D gels enables complete analysis of plasma/serum proteome and thereby allows for the discovery of novel biomarkers that might be useful in veterinary medicine.Keywords : Proteome, high-abundance protein depletion, 2-dimensional electrophoresis, farm animals