- Turkish Journal of Veterinary and Animal Sciences
- Vol: 38 Issue: 5
- Repeated superovulation treatments in Kivircik ewes during breeding and nonbreeding seasons
Repeated superovulation treatments in Kivircik ewes during breeding and nonbreeding seasons
Authors : Burcu Üstüner, Selim Alçay, Yavuz Nak, Zekariya Nur
Pages : 480-484
Doi:10.3906/vet-1401-20
View : 8 | Download : 4
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :The present study compared superovulation and embryo recovery rates applied repeatedly during breeding and nonbreeding seasons in the same 20 Kivircik sheep. Trials 1 and 3 were performed during the breeding season and the trial 2 was performed in the nonbreeding season. We synchronized the sheep's estrus cycles and induced a superovulatory response with porcine follicle stimulating hormone (pFSH) injections. Natural mating took place 12 h after the last FSH injection. The embryos were collected by laparotomy 7 days after natural mating. There was no significant difference between the number of corpora lutea (CL) counted following the 1st (breeding) and 2nd (nonbreeding) superovulatory treatments (P > 0.05). However, the number of CL in the 3rd (breeding) treatment was dramatically lower than that in the other groups (P < 0.001). Recovery rates showed significant differences among embryo flushing treatments (P < 0.001). The number of morulae + blastocysts and freezable-quality embryos decreased considerably for the 3rd flushing treatment (P < 0.001), while no significant difference was observed between the 1st and 2nd flushing treatments (P > 0.05). In conclusion, our study demonstrated that 2 consecutive in vivo embryo production programs can be successfully applied in Kivircik ewes with suitable superovulation treatments and operation techniques, irrespective of the season.Keywords : Sheep, superovulation, embryo, FSH