- Turkish Journal of Veterinary and Animal Sciences
- Vol: 23 Issue: 2
- Isolation and Preparation of Monoclonal Antibody to Ovine Immunoglobulin M
Isolation and Preparation of Monoclonal Antibody to Ovine Immunoglobulin M
Authors : Hüseyin YILMAZ, Aysan TANTAŞ, Atilla ILGAZ
Pages : 135-140
View : 3 | Download : 3
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :Ovine immunoglobulin M was isolated from sheep serum using gel filtration and affinity chromatography. Polyclonal and monoclonal antibodies against this isotype were prepared. The specificity of the monoclonal antibody was investigated using SDS-Page, immunoblotting, an enzyme-linked immunosorbent assay (ELISA) and affinity chromatography. On immunoblotting, monoclonal anti-IgM reacted with a 70 kD protein which corresponded to ovine IgM.Keywords : IgM, Isolation, monoclonal, antibody.