- Turkish Journal of Veterinary and Animal Sciences
- Vol: 24 Issue: 2
- Localization of Haemophilus somnus Antigen by an Immunoper oxidase Technique in Pneumonic Bovine Lun...
Localization of Haemophilus somnus Antigen by an Immunoper oxidase Technique in Pneumonic Bovine Lungs
Authors : Rıfkı Haziroğlu
Pages : 177-180
View : 8 | Download : 8
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :One hundred pneumonic lungs from calves were examined by an immunoper oxidase technique using avidin-biotin- per oxidase complex method in formalin-fixed, paraffin-embedded sections for Haemophilus somnus. In the histological examination of tissue sections, strong positive reactions against H. somnus were detected in 14% of the lungs. H. somnus antigens were observed at the surface and/or within the epithelial cells, macrophages, necrotic alveolar walls, alveolar -bronchial/br onchiolar exudates, fibrins, distended interlobular septa and perivascular areas. In addition, dense antigen accumulations were detected in the necrotic macrophages and neutrophils.Keywords : Haemophilus somnus, Immunoper oxidase, Pneumoniae.