- Turkish Journal of Veterinary and Animal Sciences
- Vol: 25 Issue: 4
- Changes in the Size Distribution of Steroidogenic and Non-steroidogenic Bovine Luteal Cells During t...
Changes in the Size Distribution of Steroidogenic and Non-steroidogenic Bovine Luteal Cells During the Oestrus Cycle
Authors : Arzu Y???t, ?evket Arikan
Pages : 545-550
View : 5 | Download : 3
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :The aim of this study was to investigate the size distribution of steroidogenic and non-steroidogenic luteal cells throughout the oestrus cycle. Twelve corpora lutea collected from different stages of the oestrus cycle were used. Corpora lutea collected from nonpregnant cows were dissociated into single-cell suspensions by enzyme treatments. Cells were stained for 3b-hydroxysteroid dehydrogenase (3b-HSD) activity, a marker for steroidogenic cells. The sizes of 3b-HSD positive (steroidogenic) and negative cells (nonsteroidogenic) were measured with an ocular micrometer. Very small cells (Keywords : Bovine, 3b-HSD, luteal cells, oestrus cycle