- Turkish Journal of Veterinary and Animal Sciences
- Vol: 26 Issue: 2
- Comparing the Birth Rates of Microinjected Hybrid Mouse Zygotes Transferred into Outbred and Hybrid ...
Comparing the Birth Rates of Microinjected Hybrid Mouse Zygotes Transferred into Outbred and Hybrid Foster Mothers
Authors : Haydar BAĞIŞ
Pages : 329-333
View : 5 | Download : 3
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :The purpose of this study was to compare the birth rates after transfers of microinjected hybrid mouse zygotes into hybrid CB6F1 (C57BL/6J X BALB/c) and outbred CD-1 foster mothers. One or two hours after microinjection of a b -actin-Gfp gene construct into the male pronucleus, hybrid mouse zygotes were transferred into CB6F1 and CD-1 foster mothers. In addition, non-microinjected hybrid zygotes were transferred into foster mothers of both strains. The overall data demonstrate that outbred CD-1 foster mothers are a better choice for transfer of microinjected hybrid mouse embryos, and there was a statistical difference between the transfers of microinjected zygotes into CB6F1 and CD-1 foster mothers (PKeywords : Microinjection, pronuclear stage, embryo transfer, hybrid, outbred