- Turkish Journal of Veterinary and Animal Sciences
- Vol: 26 Issue: 2
- Membrane Specific Carbonic Anhydrase (CA-IV) Expression in Bovine Lung: The Effects of Alcoholic and...
Membrane Specific Carbonic Anhydrase (CA-IV) Expression in Bovine Lung: The Effects of Alcoholic and Non-Alcoholic Drinks
Authors : Nazan Demir, Hayrunnisa Nadaroğlu
Pages : 227-233
View : 22 | Download : 6
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :Carbonic anhydrase (CA) (carbonate hydrolyase: E. C. 4.2.1.1) from bovine lung was purified by a new method and characterized. The purification level was 4306-fold. The optimum temperature for maximum enzyme activity was 37.5°C. The optimum pH was 7.4, varying between 3.5 and 10.0. SDS-polyacryamide gel electrophoresis (3-10% discontinuous SDS-PAGE) showed two distinct bands for CA-IV. The molecular weights of the enzymes were found to be approximately 54.000 and 29.000, respectively. Vmax and KM values were calculated with p-nitrophenyl acetate as substrate (166.7 µmol/L*min, 5.59*10-2 M, respectively). Changes in enzyme activity were determined in the presence of some chemicals: sulfanilamide, NaN3, KSCN, glucose, nicotine, nicotinamide, caffeine, CuSO4 and MnCl2; and some drinks: brandy, vodka, raki, whisky, cognac, beer, martini, apple liqueur, red wine, Pepsi, Yedigün, 7UP, Vimto and Coca-Cola (carbonated and non-carbonated).Keywords : Carbonic anhydrase (IV), Alcohol, Nicotine, Nicotinamide, Caffeine