- Turkish Journal of Veterinary and Animal Sciences
- Vol: 26 Issue: 3
- The Relationship Between Ascorbic Acid, Oestradiol 17 b and Progesterone in Plasma and Ovaries in Pr...
The Relationship Between Ascorbic Acid, Oestradiol 17 b and Progesterone in Plasma and Ovaries in Pregnant Holstein Cows
Authors : Seyfullah Haliloğlu, Behiç Serpek, Nuri Başpinar
Pages : 639-644
View : 20 | Download : 5
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :This study was performed to determine the relationship between the corpus luteum weight/diameter, follicular diameter and the levels of vitamin C, progesterone and oestradiol 17-b of the plasma, corpus luteum and follicular fluid of pregnant Holstein cows in a slaughterhouse. Progesterone and oestradiol 17-b concentrations were determined by the microtitration plate EIA method. Vitamin C levels were determined by spectrophotometer. Although there was a positive correlation between follicular fluid oestradiol 17-b levels and the follicle diameter in the follicular fluid, in contrast to oestradiol 17-b levels, small follicles have higher progesterone content than large follicles. This negative correlation was due to the fact that progesterone is the precursor of oestradiol 17-b. There was no correlation between corpus luteum progesterone levels and corpus luteum weight/diameter. However, a positive correlation between corpus luteum progesterone levels and the corpus luteum weight/diameter was observed; therefore, we suggest that this situation resulted from placenta-originated progesterone synthesis in pregnancy. Furthermore, levels of corpus luteum vitamin C were found to be higher and we concluded that this increase resulted from the effect of vitamin C on steroid and collagen biosynthesis. Nevertheless, negative correlations between corpus luteum vitamin C concentrations and corpus luteum weight/diameter were seen, and hence we suggest that this may be the result of the inhibitory effect of vitamin C on PGF2a synthesis.Keywords : Pregnancy, vitamin C, steroid hormones, ovary, cow