- Turkish Journal of Veterinary and Animal Sciences
- Vol: 27 Issue: 1
- Development of an Enzyme Immunoassay for the Determination of Testosterone
Development of an Enzyme Immunoassay for the Determination of Testosterone
Authors : Bülent Güven
Pages : 45-50
View : 6 | Download : 3
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :In this study, a double antibody enzyme immunoassay for the direct determination of testosterone in plasma was developed. Testosterone 3-O-CMO was conjugated with horseradish peroxidase by the mixed anhydride method, and the conjugate purified by column chromatography (sephadex G-25). Testosterone antibody was obtained by immunization of rabbits against Testosterone-3-O-CMO-BSA. Cross reactions of the antiserum against some steroids were found to beKeywords : Testosterone, enzyme immunoassay