- Turkish Journal of Veterinary and Animal Sciences
- Vol: 27 Issue: 2
- Optimisation of UV Treatment Duration to Induce Haploid Androgenesis in the Nile tilapia (Oreochromi...
Optimisation of UV Treatment Duration to Induce Haploid Androgenesis in the Nile tilapia (Oreochromis niloticus L.)
Authors : Ismihan Karayücel, Sedat Karayücel
Pages : 401-407
View : 12 | Download : 3
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :The optimum UV duration times of eggs were examined in order to develop a simple and safe method for inducing androgenetic development in the Nile tilapia, Oreochromis niloticus. The yield of androgenetic haploid O. niloticus to the pigmentation stage was 18.53 ± 5.3% (relative to controls) with an optimal UV irradiation dose of 540 Jm-2 (at 150 µWcm-2 ) for 6 min. Most embryos developing after fertilisation with normal spermatozoa showed abnormal morphology and a haploid number of chromosomes (n = 22). The success of oocyte denucleation was also assessed by using the recessive "blond" skin pigmentation character.Keywords : Androgenesis, chromosome manipulations, Nile tilapia, Oreochromis niloticus