- Turkish Journal of Veterinary and Animal Sciences
- Vol: 28 Issue: 3
- Production of Chimeric Cattle Embryos by Reaggregation of Blastomeres Obtained from Day 4 Bovine Emb...
Production of Chimeric Cattle Embryos by Reaggregation of Blastomeres Obtained from Day 4 Bovine Embryos
Authors : Hakan Sağirkaya
Pages : 623-631
View : 7 | Download : 4
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :The aim of this study was to investigate the possibility of producing chimeric bovine embryos by reaggregating blastomeres of disaggregated day 4 bovine embryos. Oocytes were obtained from a slaughterhouse. Selected oocytes were matured, fertilized and cultured in vitro. Fertilization day was taken as day 0. Blastomeres of day 4 embryos were disaggregated and then pooled together. Chimeric embryos were randomly constructed by reaggregating blastomeres in empty zonae pellucidae. Non-chimeric handled control embryos were produced by transferring blastomeres from each embryo into their own empty zonae pellucidae. A total of 79 day 4 cattle embryos were used for disaggregation of blastomeres, and 27 chimeric and 12 non-chimeric handled control embryos were obtained from them. The rates of blastocyst formation of chimeric and non-chimeric handled control embryos were 40.7% (11/27) and 50.0% (6/12), respectively, and the difference between them was not statistically important (P > 0.05). These results showed the possibility of producing chimeric cattle embryos by reaggregating blastomeres obtained from day 4 embryos.Keywords : Embryo, in vitro fertilization, chimera, cattle