- Turkish Journal of Veterinary and Animal Sciences
- Vol: 29 Issue: 2
- Cloning of Nucleoprotein Gene (NP) of Rinderpest Virus (RPV) RBOK Vaccine Strain
Cloning of Nucleoprotein Gene (NP) of Rinderpest Virus (RPV) RBOK Vaccine Strain
Authors : ?ükrü Tonbak, Aykut Özdarendel?, Yusuf Bolat
Pages : 297-301
View : 6 | Download : 4
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :In the present study cloning of the NP gene in a cloning vector is reported. For this purpose, Vero cells were infected with rinderpest RBOK vaccine strain. Total RNA was obtained from the infected cells and reverse-transcription (RT) was performed to get cDNAs of NP gene. Afterwards, the NP gene was amplified by polymerase chain reaction (PCR) with the primers specific to the NP gene. The NP gene, 1575 bp in length, was cloned into pGEM-3Zf (-) cloning vector. PCR screening and enzyme digestion assays carried out to verify the presence of the NP gene.Keywords : Rinderpest virus, nucleoprotein gene, cloning