- Turkish Journal of Veterinary and Animal Sciences
- Vol: 35 Issue: 1
- Association analysis of aquaporin 7 (AQP7) gene variants with semen quality and fertility in bulls
Association analysis of aquaporin 7 (AQP7) gene variants with semen quality and fertility in bulls
Authors : Teng-he Ma, Ji-feng Liu, Rui-feng Zhao, Hao Jiang, Li-sheng Dai, Yu-min Zhao, Zhi-hui Zhao, Jia-bao Zhang
Pages : 63-66
View : 7 | Download : 2
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :Aquaporin 7 (AQP7) gene as a candidate Antifreeze gene was investigated and associated with fresh and frozen semen quality traits in 45 Simmental and Charolais bulls. PCR-SSCP, PCR-RFLP, and sequencing method were employed to detect the single nucleotide polymorphisms (SNPs) of the AQP7 gene. Two mutations, A264G and G371C, were identified. The allele frequencies were A (0.97, 0.96) to B (0.03, 0.04) and C (0.97, 0.91) to D (0.03, 0.09) in Simmental and Charolais bulls, respectively. A264G substitution was significantly associated with acrosome integrity ratio (P < 0.01) and motility (P < 0.05) in frozen semen. Meanwhile, G371C substitution was significantly associated with acrosome integrity ratio (P < 0.05), percentages of viable sperm (P < 0.05), and motility (P < 0.01) in frozen semen. The results prove that AQP7 gene SNPs are useful for marker-assisted selection in early seed selection in the bull industry.Keywords : Key words: AQP7 gene, single nucleotide polymorphisms, SNPs, association analysis, Simmental bulls, Charolais bulls