- Turkish Journal of Veterinary and Animal Sciences
- Vol: 35 Issue: 1
- Stage-specific developmental gene expression of goat preimplantation embryos produced in vitro
Stage-specific developmental gene expression of goat preimplantation embryos produced in vitro
Authors : Yong-jun Li, Hong Ao, Gui-jin Sun, Li Zhang
Pages : 15-19
View : 8 | Download : 3
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :Single preimplantation embryo mRNA differential display was used to study the gene expression of 2-cell,4-cell, and 8-16-cell stage goat embryos produced in vitro, and 16 different stage specific bands in total were screened. In these specific bands, 5 were in 2-cell stage, 4 were in 4-cell stage, and 7 were in 8-16-cell stage, respectively. The sequencing and alignment results suggested that 4 differential expression bands were homologous with human petidylarginine deiminase (PAD), bovine insulin-like growth factor binding protein-3 (IGFBP3), bovine NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 4 (NDUFA4), and dog cyclin B3 (CCNB3) genes, respectively. All these 4 genes had the functions on physiological regulation and were important factors in the process of goat preimplantation embryo development.Keywords : Key words: Single preimplantation embryo mRNA differential display, goat, embryo, gene