- Turkish Journal of Medical Sciences
- Vol: 45 Issue: 4
- Esophageal replacement by hydroxylated bacterial cellulose patch in a rabbit model
Esophageal replacement by hydroxylated bacterial cellulose patch in a rabbit model
Authors : Changlai Zhu, Fang Liu, Wenbo Qian, Yingjie Wang, Qingsheng You, Tianyi Zhang, Feng Li
Pages : 762-770
Doi:10.3906/sag-1407-140
View : 9 | Download : 5
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :Background/aim: To repair esophageal defects by hydroxylated and kombucha -synthesized bacterial cellulose (HKBC) patch in a rabbit model. Materials and methods: Semicircular esophageal defects 1 cm in length of the cervical esophagus were initially created in 18 Japanese big-ear rabbits and then repaired with HKBC patch grafts. The clinical outcomes including survival rate, weight change, food intake, and hematological and radiologic evaluation were observed. After X-ray evaluation, the rabbits were sacrificed sequentially at 1, 3, and 6 months for histopathologic analysis with light microscopy and scanning electron microscopy. Results: Survival rate during the first month was 88.9% (n = 16). Two rabbits died from anastomotic leakage during the entire follow-up. Postoperatively, feeding function and body weight were gradually restored in the surviving animals. No hematological abnormalities were found, and no obvious anastomotic leakage, stenosis, or obstruction was observed under X-ray examination. The histopathologic results showed a progressive regeneration of the esophagus in the graft area, where the neo-esophagus tissue had characteristics similar to native esophageal tissue after 3 months of surgery. Conclusion: HKBC is beneficial for esophageal tissue regeneration and may be a promising material for esophageal reconstruction.Keywords : Artificial esophagus, kombucha, bacterial cellulose, biological material