- Turkish Journal of Medical Sciences
- Vol: 45 Issue: 1
- A molecular epidemiological investigation of multistate outbreaks of Salmonella Enteritidis from cli...
A molecular epidemiological investigation of multistate outbreaks of Salmonella Enteritidis from clinical and environmental samples in Turkey, 2000-2010
Authors : Sümeyra Acar, Belkıs Levent, Ekrem Atalan
Pages : 76-83
Doi:10.3906/sag-1401-33
View : 14 | Download : 4
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :To analyze interrelated Salmonella Enteritidis strains isolated in Turkey. Fifty-five S. Enteritidis surveillance strains were isolated from human feces and environmental samples from different regions in Turkey between 2000 and 2010. Materials and methods: Clinical isolates were selected from different outbreaks in the Turkish National Reference Laboratory. All isolates were submitted to antimicrobial susceptibility test, plasmid profile analysis, and XbaI-digested pulsed-field gel electrophoresis. Results: The strains were scanned against 20 antibiotics and for 3 of them (amikacin, ciprofloxacin, gentamicin), all strains were found to be sensitive. Five isolates had no plasmid. Most of test strains carried the 57-kb plasmid in common and 15 genotypes were identified among the 55 isolates. Six genotypes were related closely, 3 genotypes were undistinguished, and 6 genotypes were unrelated. Conclusion: To our knowledge, this is the first report on the phenotypic and molecular characterization of S. Enteritidis isolates from both environmental samples and clinical isolates in Turkey.Keywords : Salmonella Enteritidis, plasmid, pulsed-field gel electrophoresis, antimicrobial sensitivity