- Turkish Journal of Medical Sciences
- Vol: 28 Issue: 1
- The Effects of Phenoxybenzamine on Tyrosine Hydroxylase (TH) and TH mRNA Level in Adrenal Medulla of...
The Effects of Phenoxybenzamine on Tyrosine Hydroxylase (TH) and TH mRNA Level in Adrenal Medulla of Sprague Dawley Rats
Authors : Muhittin Yürekli
Pages : 35-40
View : 9 | Download : 5
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :The effects of anti-hipertensive phenoxybenzamine were in-vestigated on tyrosine hydroxylase (TH) enzyme activity and TH mRNA levels. In the present study 5 months male sprague dawley (SD) rats were used. Phenoxybenzamine was injected i.p as 20 mg/kg which prepared in the 0.9 % NaCl and 5% ethanole. TH activity was measured by detecting of formation of 3 H 2 O as a formation of dopa from 3 H-tyrosine by radioisotope technique total adrenal RNA was isolated and hybridized wit 32 P labeled cDNA. TH mRNA was assayed by densitometric scanning of the autoradiograms using a densitometer. TH activity and TH mRNA levels were found to be significantly increased by the effcet of phenoxybenzamine (PKeywords : Phenoxybenzamine, tyrosine hydroxylase, adrenal medulla, TH mRNA