- Turkish Journal of Medical Sciences
- Vol: 31 Issue: 3
- Flow Cytometric Analysis of the Effects of Methotrexate and Vepesid on the HEp-2 Cell Cycle
Flow Cytometric Analysis of the Effects of Methotrexate and Vepesid on the HEp-2 Cell Cycle
Authors : Nizami Duran
Pages : 187-192
View : 7 | Download : 3
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :Objective: To determine the mechanism of action of Methotrexate and Vepesid on the HEp-2 cells isolated from human laryngeal cancer cells morphologically and flow cytometrically (G1, G2, S and PI). Materials and Methods: The HEp-2 continuous cell line was used. Cultivation of the cells was realized in EMEM medium with 10% fetal bovine serum at an atmosphere of 37ºC with 5% CO2. Six different concentrations of Vepesid and Methotrexate were prepared by diluting with deionized water (5 µg/ml, 50 mg/ml, 500 µg/ml). The morphological and cell cycle parameters of HEp-2 cells were determined by inverted microscope and flow cytometer respectively. Results: In the morphological examination, Vepesid was found to have a more significant cytopathologic effect on the cells than Methotrexate, whereas in the flow cytometric examination, it was found that whilst Methotrexate stopped the cells at the S and G2 phases, Vepesid did that only at the G1 phase. Conclusion: Both the flow cytometric and cell morphological analysis showed Vepesid to be more effective than Methotrexate on HEp-2 cells. Results of studies conducted show the mechanism of action of these drugs to be dependent on the origin of the cell and on the drug type.Keywords : Cell cycle, Methotrexate, Vepesid, HEp-2 cell, flow cytometer