- Turkish Journal of Medical Sciences
- Vol: 40 Issue: 3
- Molecular analyses of Salmonella serotype Typhi and Salmonella serotype Paratyphi B strains isolated...
Molecular analyses of Salmonella serotype Typhi and Salmonella serotype Paratyphi B strains isolated in Turkey
Authors : Iştar Dolapçi, Birsel Erdem, Alper Tekeli, Ebru Us, Mehseti Bayramova, Begüm Saran, Fikret Şahin
Pages : 447-458
View : 7 | Download : 3
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :To investigate the characteristics of Salmonella serotype Typhi and Salmonella serotype Paratyphi B, which still remain significant in Turkey. Materials and methods: Eighteen S. Typhi and 42 S. Paratyphi B strains isolated from clinical samples were investigated by plasmid profile analysis, enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR), and pulsed field gel electrophoresis (PFGE) methods. Results: Plasmid profile analysis showed that 10 out of 18 (56%) S. Typhi isolates harbored 1 to 3 plasmids, and 11 out of 42 (26.2%) S. Paratyphi B isolates harbored 1 to 4 plasmids. PFGE performed with restriction enzyme AvrII grouped both S. Typhi and S. Paratyphi B isolates into 14 clusters; with restriction enzyme XbaI, S. Typhi and S. Paratyphi B isolates were grouped into 13 and 12 clusters, respectively. Conclusion: These results showed that plasmid profile analysis and PFGE are reliable and discriminative methods complementing the phenotypic characters of S. Typhi and S. Paratyphi B isolates, and may contribute to the epidemiologic investigation of typhoid or paratyphoid diseases. The study presented here is the first report on S. Typhi and S. Paratyphi B isolates from Turkey investigated by plasmid profile analysis, ERIC-PCR, and PFGE methods.Keywords : Key words: Salmonella serotype Typhi, Salmonella serotype Paratyphi B, plasmid profiles, ERIC-PCR, PFGE, Turkey