- Turkish Journal of Medical Sciences
- Vol: 42 Issue: 2
- Thoracic epidural anesthesia with bupivacaine attenuates systemic inflammatory response and lung imp...
Thoracic epidural anesthesia with bupivacaine attenuates systemic inflammatory response and lung impairment associated with cerulein-induced pancreatitis in rats
Authors : Nurdan Bedirli, Nalan Akyürek, Bahadır Kösem, Melek Yaman
Pages : 217-227
View : 18 | Download : 6
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :To evaluate the effects of thoracic epidural anesthesia (TEA) on acute pancreatitis (AP)-induced lung injury in a rat model. Materials and methods: A total of 24 rats were divided into 3 groups: the control group (n = 8; pancreatitis), the bupivacaine group (n = 8; pancreatitis + 20 µL/h 0.5% bupivacaine), and the saline group (n = 8, pancreatitis + 20 µL/h saline). Pancreatitis was induced by 4 intraperitoneal injections of 50 µg/kg cerulein. At 24 h, blood was collected for determination of amylase, blood gas, tumor necrosis factor-a (TNF-a), interleukin (IL)-6, and IL-1b; lung samples were collected for the measurement of glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT), and myeloperoxidase (MPO) concentrations and for immunohistochemical examinations, apoptosis determination, and wet/dry ratio determinations. Pancreas samples were collected for the histological investigation to confirm AP. Results: Serum amylase decreased significantly in the bupivacaine group when compared to the control and saline groups. Bupivacaine showed a significant decrease in the TNF-a and IL-1b concentrations and an increase in the GPx and SOD concentrations compared to both the saline and control groups. The wet/dry ratio showed a significant difference between the control and bupivacaine groups. Bupivacaine significantly decreased the injury score, apoptosis, and ICAM-1 secretion in the lung tissue. Conclusion: The present study demonstrated that TEA with bupivacaine decreased the systemic inflammation and lung injury due to AP.Keywords : Key words: Thoracic epidural anesthesia, acute pancreatitis, lung damage