- Turkish Journal of Medical Sciences
- Vol: 43 Issue: 5
- One single-tube PCR assay to detect both CD17 (A>T) and IVS-II nt-654 (C>T) homozygous mutations of ...
One single-tube PCR assay to detect both CD17 (A>T) and IVS-II nt-654 (C>T) homozygous mutations of b-thalassemia
Authors : Qi Liu, Jun-li Wang, Guo-can Yang, Xue-jun Dong, Zong-xin Wang, Guo-xing Ge, Xiao-feng Gu
Pages : 764-767
Doi:10.3906/sag-1209-60
View : 8 | Download : 3
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :Several methods can be used to detect gene mutations associated with b-thalassemia, but it is difficult to achieve reliable and reproducible results. In this study, we introduce a new method, a single-tube multiplex polymerase chain reaction (PCR) assay, to detect both CD17 (A>T) and IVS-II nt-654 (C>T) homozygous mutations. Materials and methods: This new method designs specific primers to diagnose homozygous mutations and normal controls. Results: After PCR amplification, homozygous mutations produce different fragments from normal controls. Conclusion: This study represents an important step towards the development of a novel protocol to diagnose b-thalassemia and other diseases that target numerous mutations.Keywords : Key words: b-Thalassemia, single-tube, CD17, IVS-II nt-654