- Turkish Journal of Chemistry
- Vol: 43 Issue: 6
- Synthesis, characterization, and investigation of singlet oxygen, DNA interaction, and topoisomerase...
Synthesis, characterization, and investigation of singlet oxygen, DNA interaction, and topoisomerase I inhibition properties of novel zinc(II) phthalocyanine
Authors : Ümit Demirbaş
Pages : 1646-1655
View : 9 | Download : 5
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :In this work, phthalonitrile (3) and zinc(II) phthalocyanine (4) were prepared. To determine the photodynamic therapy potential of compound 4, singlet oxygen quantum yield, DNA binding and cleavage, and topoisomerase I inhibition experiments were performed. The singlet oxygen quantum yield value of compound 4 was found higher than that of the standard unsubstituted zinc(II) phthalocyanine compound (Std-ZnPc). The binding experiments showed that compound 4 interacted with ct-DNA strongly via nonintercalation mode. pBR322 plasmid DNA cleavage activity of the compound was investigated using agarose gel electrophoresis. The results showed that the compound 4 had important DNA cleavage activity. The E. coli DNA topoisomerase I inhibition effects of compound 4 were investigated using agarose gel electrophoresis. Compound 4 had an E. coli topoisomerase I inhibitory effect at increasing concentrations. The results showed that compound 4 has photosensitizer potential in photodynamic therapy.Keywords : DNA-binding, topoisomerase I, DNA-photocleavage, phthalocyanine