- Turkish Journal of Chemistry
- Vol: 26 Issue: 4
- The Simultaneous Determination of Quinoline Yellow (E-104) and Sunset Yellow (E-110) in Syrups and T...
The Simultaneous Determination of Quinoline Yellow (E-104) and Sunset Yellow (E-110) in Syrups and Tablets by Second Derivative Spectrophotometry
Authors : Mahmure Üstün Özgür
Pages : 501-508
View : 9 | Download : 5
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :A very simple spectrophotometric method using measurements at zero-crossing wavelength is described for resolving binary mixtures of the food dyes, Sunset Yellow (E-110) and Quinoline Yellow (E-104). Calibration graphs are linear up to 15.0 m gml-1 of Sunset Yellow (r = 0.9998), and Quinoline Yellow (r = 0.9999). The assay procedure for E-110 and E-104 in pharmaceutical products involves the extraction of the dyes with AcOH/NaOAc buffer solution (pH = 4.5), filtration, and measurement of the second derivative absorbance values at 410.0 nm for E-104 and 533.1 nm for E-110. This method was used for determining synthetic mixtures of these dyes in different ratios and it was successfully applied to two commercial products without a previous separation step.Keywords : Dyes, derivative spectrophotometry, pharmaceutical products, Sunset Yellow, Quinoline Yellow