- Turkish Journal of Chemistry
- Vol: 38 Issue: 2
- Fluorescence quenching study of moxifloxacin interaction with calf thymus DNA
Fluorescence quenching study of moxifloxacin interaction with calf thymus DNA
Authors : Yun-kai Lv, Pan Li, Miao-lun Jiao, Bao-sheng Liu, Chao Yang
Pages : 202-209
Doi:10.3906/fiz-1207-21
View : 9 | Download : 6
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :Moxifloxacin (MOX) is a fourth-generation synthetic fluoroquinolone antibacterial agent with many important therapeutic properties. Fluorescence quenching was used to study the interaction of MOX with calf thymus DNA (ct-DNA) in aqueous solution. The intercalative binding mode and a static quenching mechanism were confirmed by the Stern--Volmer quenching rate constant (Kq) of 3.48 \times 1011 M-1 s-1 at 298 K. The thermodynamic parameters (D H = --118.4 KJ mol-1 and D S = --299.4 J mol-1 K-1) were calculated at different temperatures, and they indicate that the main forces between MOX and ct-DNA are hydrogen bonding and Van der Waals force. We proved at the same time the presence of one single binding site on ct-DNA, and the binding constant is 1.28 \times 105 M-1 at physiological pH. The results may provide a basis for further studies and clinical application of antibiotics drugs.Keywords : Moxifloxacin, ct-DNA, fluorescence quenching, binding