- Turkish Journal of Biology
- Vol: 43 Issue: 4
- A magnetic affinity approach to identify plant GABA-binding proteins
A magnetic affinity approach to identify plant GABA-binding proteins
Authors : Jie Zou, Jingzhe Guo, Shisheng Li
Pages : 246-255
View : 8 | Download : 3
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :In plants, GABA plays a critical role in sexual plant reproduction; however, GABA receptors and the associated detailed signaling mechanisms remain to be elucidated. Our experiments show that the proposed technique is reliable and convenient for probing GABA-binding proteins and could be applicable in similar projects by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl). New probes produced by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl) can obtain useful information on GABA receptors in plants.Keywords : GABA, receptor, magnetic bead, signaling, pollen