- Turkish Journal of Biology
- Vol: 42 Issue: 2
- Expression of a bacterial aroA gene confers tolerance to glyphosate in tobacco plants
Expression of a bacterial aroA gene confers tolerance to glyphosate in tobacco plants
Authors : Feng Liu, Yueping Cao
Pages : 187-194
View : 7 | Download : 3
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :Glyphosate is a widely used herbicide that inhibits the 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS)-encoding aroA gene in the shikimate pathway. The discovery and cloning of the aroA gene with high resistance is central to breeding a transgenic glyphosate-resistant plant. A novel aroA Pantoea gene from Pantoea G-1 was previously isolated and cloned. The aroA Pantoea enzyme was defined as a new class I EPSPS with glyphosate resistance. The aroA Pantoea gene was introduced into tobacco through Agrobacterium -mediated transformation. The transgenic tobacco plants were confirmed by PCR, RT-PCR, and Southern blot. The analysis of glyphosate resistance also showed that the transgenic tobacco plants could survive at 15 mM glyphosate; the glyphosate resistance level of the transgenic plants is higher than the agricultural application level recommended by most manufacturers. Overall, this study shows that aroA Pantoea can be used as a candidate gene for the development of genetically modified crops.Keywords : aroA, glyphosate, Pantoea, tobacco, transformation