- Turkish Journal of Biology
- Vol: 40 Issue: 5
- Baculoviral vector loaded mesenchymal stem cells as efficient gene therapy tools for cancer treatmen...
Baculoviral vector loaded mesenchymal stem cells as efficient gene therapy tools for cancer treatment
Authors : Sedef Hande Aktaş, Hakan Akbulut, Ayşe Eser Elçin, Mahmut Parmaksiz, Arzu Keskin Aktan, Arzu Çöleri Cihan, Yaşar Murat Elçin, Fikri Içli
Pages : 1121-1128
View : 7 | Download : 3
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :Insufficient targeting of the therapeutic genes to tumor cells is one of the major reasons for failure in cancer gene therapy. Mesenchymal stem cells (MSCs) seem to be a good candidate as a carrier for gene therapy because of its selective tumor tissue-homing properties. In the current study, we constructed baculoviral vectors (BVs) carrying cytosine deaminase (CD) (BV-CD) or green fluorescence protein (GFP) genes (BV-GFP) and tested the transduction efficiency of the vectors in tumor and mesenchymal stem cells. We also tested the in vivo efficacy of the BV-CD vector in a colon cancer model. Our results showed that the recombinant baculoviral vectors can efficiently transduce mammalian cells and express genes of interest. The BV-CD vector treatment caused significant in vitro cytotoxicity when used with 5-fluorocytosine. MSCs loaded with the BV-CD vector caused a significant delay in tumor growth and increased survival when compared to control and MSC alone treated groups bearing colon cancer.Keywords : Our results show that the recombinant BV-CD vector could be used either alone or loaded into MSCs in the treatment of established tumors.