- Turkish Journal of Biology
- Vol: 40 Issue: 3
- Molecular cytogenetic identification of a novel hexaploid wheat-Thinopyrum intermedium partial amphi...
Molecular cytogenetic identification of a novel hexaploid wheat-Thinopyrum intermedium partial amphiploid with high protein content
Authors : Mariyana Georgieva, Klaudia Kruppa, Nedyalka Tyankova, Marta Molnar Lang
Pages : 554-560
View : 10 | Download : 4
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :Genomic in situ hybridization and fluorescence in situ hybridization were used to define the cytogenetic constitution of the intergeneric introgression line 29(1-57) obtained from the wheat × Thinopyrum intermedium (wheatgrass) cross. This line is a high-protein line resistant to leaf rust, yellow rust, and powdery mildew under field conditions and has 42 chromosomes. GISH analysis using a total genomic DNA probe from Thinopyrum intermedium resulted in the identification of 14 chromosomes of wheatgrass origin. The sequential GISH patterns obtained using St-genomic and J-genomic DNA as probes and ABD-genomic DNA from wheat as a blocker led to a better determination of the genomic type of wheatgrass chromosomes. Rearrangements involving Thinopyrum chromosomes and the A, B, and D genomes of wheat were not observed. FISH using the repetitive DNA probes pSc119.2, Afa-family, and pTa71 allowed for identification of all the wheat chromosomes present.Keywords : FISH, GISH, wheat-Thinopyrum intermedium introgression line