- Turkish Journal of Biology
- Vol: 22 Issue: 2
- Purification and Characterization of Pediocin F, A Bacteriocin Produced By Pediococcus acidilacticiF
Purification and Characterization of Pediocin F, A Bacteriocin Produced By Pediococcus acidilacticiF
Authors : Özlem Osmanagaoğlu
Pages : 217-228
View : 6 | Download : 3
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :In this work, Pediococcus acidilactici F isolated from fermented sausage was reported to produce an extracellular antimicrobial peptide, termed Pediocin F, that was protein in nature and effec-tive against many bacteria associated with food spoilage and food related health hazards. Pediocin F is reported here to be sensitive to proteolytic enzymes, resistant to heat and organic solvents, and active over a wide range of pH. We have also reported that the extracellular antimicrobial protein pro-duced by P. acidilactici F was really a small peptide. Coomassie-blue stained SDS-PAGE technique allowed us to estimate the molecular weight of this small peptide to be about 4460 dalton.Keywords : Pediococcus acidilactici, Pediocin, characterization, SDS-PAGE