- Turkish Journal of Biology
- Vol: 23 Issue: 2
- Anthranilate Synthetase From Escherichia coli SJHI: Purification and Some Properties
Anthranilate Synthetase From Escherichia coli SJHI: Purification and Some Properties
Authors : Münevver Arisoy
Pages : 169-176
View : 6 | Download : 3
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :A procedure employed in the purification of anthranilate synhetase of Escherichia coli SJHI is described. The purified anthranilate synthetase appeared to be homogeneous when examined with poliacrylamide gel electrophoresis. Phenly-sepharose CL-4B and Blue dye sepharose were used for purification. A positive correlation was found between purification and ammonium sulfate especially using concentrations greater than 10%. A positive effect 2-Mercapto-ethanol on the enzyme stability was also determined.Keywords : Purification, Anthranilate synthetase, Escherichia coli SJHI.