- Turkish Journal of Biology
- Vol: 24 Issue: 2
- Monitoring of Germin Synthesis by Pre-Absorbed Anti-Germin Serum
Monitoring of Germin Synthesis by Pre-Absorbed Anti-Germin Serum
Authors : Mahmut Çalişkan And Andrew C. Cuming
Pages : 271-278
View : 8 | Download : 4
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :The transition of a quiescent plant embryo into a vigorously growing embryo revealed some nascent synthesis of gene products, among them germin has been characterized. Germin is a water soluble homopentameric glycoprotein whose synthesis and accumulation appear to be closely related to early wheat embryo development. Germin synthesis was monitored by employing an anti-germin serum which had been pre-adsorbed with wheat extract and optimized in order to decrease nonspecific binding. Germin accumulation was shown to occur in germinating embryos from 16h up to 6 day post-imbibition. Germin as a rare protein in wheat seedlings was detected as a dark band on X-rays in amounts as small as 20 µl protein extracts. Neither germin in mature wheat plant organs nor germinated embryos were detected prior to 16 hours of imbibition.Keywords : Early Wheat development, Germin, Western blotting.