- Turkish Journal of Biology
- Vol: 25 Issue: 2
- Availability of Use of Total Extracellular Proteins in SDS-PAGE for Typing Staphylococcus aureus and...
Availability of Use of Total Extracellular Proteins in SDS-PAGE for Typing Staphylococcus aureus and Coagulase-Negative Staphylococci
Authors : Sefa C. Saçilik
Pages : 145-151
View : 8 | Download : 3
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :A total of nineteen Staphylococcus references, nine of which are Staphylococcus aureus and ten coagulase-negative Staphylococci (CNS), were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Whole-cell protein (WCP) profiles obtained by SDS-PAGE were compared with banding patterns produced by SDS-PAGE of total extracellular proteins (TEP). No report has yet been issued which explains Staphylococcus typing by SDS-PAGE of extracellular proteins. SDS-PAGE of whole-cell extracts did not usefully distinguish different isolates of Staphylococci. Our preliminary results suggest that SDS-PAGE of total extracellular proteins provides additional criteria and characteristic banding patterns for the study of the epidemiology and characterization of Staphylococci. SDS-PAGE of extracellular proteins provided a rapid, reproducible and discriminative method for characterization of Staphylococci which is easy to perform and interpret.Keywords : Staphylococcus aureus, coagulase-negative staphylococci (CNS), protein profiles, typing.