- Turkish Journal of Biology
- Vol: 26 Issue: 1
- Typing of Some Clinically Significant Nocardia Strains Using a Digoxigenin-Labelled rDNA Gene Probe
Typing of Some Clinically Significant Nocardia Strains Using a Digoxigenin-Labelled rDNA Gene Probe
Authors : Kamil Işik, Nevzat Şahin, Ergin Kariptaş
Pages : 1-8
View : 13 | Download : 5
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :In this study, ribotyping (restriction polymorphism ribosomal RNA analyses) was applied as a taxonomic tool for bacteria belonging to the genus Nocardia. A total of 21 Nocardia strains were clearly distinguished using a combination of EcoRV gene restriction endonuclease patterns together with a digoxigenin-labelled Streptomyces violaceoruber TK21 rDNA probe. Five distinct ribotypes were identified, and each ribotype contained 4 to 8 restriction fragments ranging in size from 1.2 to 20.7 kb. The evaluation of the results showed that N. brasiliensis, N. farcinica, N. otitidiscaviarum and N. seriolea are taxonomically well-established species, except N. pseudobrasiliensis. Therefore, it can be concluded that ribotyping technique provide a useful information for the classification of clinically significant Nocardia.Keywords : Nocardia, Ribotyping, Taxonomy