- Turkish Journal of Biology
- Vol: 27 Issue: 2
- Adventitious Shoot Organogenesis and Plant Regeneration from Cotyledons of Diploid Diyarbakır Waterm...
Adventitious Shoot Organogenesis and Plant Regeneration from Cotyledons of Diploid Diyarbakır Watermelon (Citrullus lanatus cv. "Sürme")
Authors : Vedat Pirinç
Pages : 101-105
View : 5 | Download : 2
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :Adventitious shoots were obtained from the diploid Diyarbakır watermelon cultivar "Sürme" by culturing cotyledons on shoot regeneration medium for 3 weeks. The effects of two cytokinins, benzyl amino purine (BA) and kinetine (Kin), on shoot organogenesis were examined. The number of shoots per explant were approximately more than 50% higher when 0.5 mg/l BA was used compared to the most effective kinetin concentration (1 mg/l). Plants were obtained by transferring 1 to 2 cm shoots to MS medium supplemented with 1-4 mg/l naphthalene acetic acid. Most of the regenerated plants (more than 50%) were successfully transplanted to the field. This demonstrates that in vitro propagation can be used to produce high quality diploid Diyarbakır watermelon for use in breeding lines.Keywords : Regeneration, Tissue culture, Watermelon