- Turkish Journal of Biology
- Vol: 35 Issue: 2
- UV-B induces cell death in the lichen Physcia semipinnata (J.F.Gmel)
UV-B induces cell death in the lichen Physcia semipinnata (J.F.Gmel)
Authors : Dilek Ünal, Yiğit Uyanikgil
Pages : 137-144
View : 10 | Download : 4
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :We have examined the consequences of Ultraviolet-B (UV-B) irradiation in the thallus of the lichen Physcia semipinnata (J.F.Gmel) in terms of cell viability and apoptotic-like formation. UV-B induced oligonucleosomal DNA fragmentation was detected by TUNEL assay and this is the first study showing DNA fragmentation in thalli. The intensity of TUNEL-positive cells after exposure to UV-B at doses up to 95.9 J/cm2 was higher in the photobiont layer than the mycobiont layer.Keywords : Key words: TUNEL reaction, acridine orange staining, UV-B radiation, lichen