- Turkish Journal of Biology
- Vol: 35 Issue: 3
- Determination of antimicrobial and antiproliferative activities of the aquatic moss Fontinalis antip...
Determination of antimicrobial and antiproliferative activities of the aquatic moss Fontinalis antipyretica Hedw.
Authors : Filiz Savaroğlu, Cansu Filik Işçen, Ayşe Pınar Öztopcu Vatan, Selda Kabadere, Semra Ilhan, Ruhi Uyar
Pages : 361-369
View : 19 | Download : 8
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :The purpose of the current study was to investigate the possible antimicrobial and anticancer properties of the aquatic moss Fontinalis antipyretica Hedw. (Fontinalaceae). We first obtained 8 different extracts (methanol; chloroform; acetone; ethyl acetate A, B, C, and D) by two different extraction processes. The antimicrobial activity of these extracts was then assessed by using the well diffusion method against 8 bacterial and 7 fungal strains. The results of antimicrobial studies showed that extracts of chloroform, acetone, and ethyl acetate A and C were active against almost all the species tested. The most effective extract was C, which was then screened through a preparative TLC (bioautography) for its active components. Spot 4 of extract C exhibited the highest antimicrobial activity against 5 bacterial strains (B. cereus, B. subtilis, E. faecalis, E. aerogenes, and Y. enterocolitica) and 3 fungal strains (A. flavus, F. solani, and F. graminearum), with MIC values of 93.8-375.0 µg/mL and 187.5-375.0 µg/mL, respectively. In addition, in vitro toxicity of the active component of extract C at the concentrations of 0.16, 1.6, 16, 80, and 160 µg/mL was tested. Depending on dose and time, only 80 and 160 µg/mL decreased rat glioma (C6) cell viability after 24 or 48 h. The present study suggests the possibility that Fontinalis antipyretica possesses antimicrobial and anticancer agent(s).Keywords : Key words: Activity, antibacterial, antifungal, Bryophyta, extract, toxicity