- Turkish Journal of Biology
- Vol: 37 Issue: 1
- Comparison of the volatiles of Daphne pontica L. and D. oleoides Schreber subsp. oleoides isolated b...
Comparison of the volatiles of Daphne pontica L. and D. oleoides Schreber subsp. oleoides isolated by hydro- and microdistillation methods
Authors : Ilhan Gürbüz, Betül Demirci, Gerhard Franz
Pages : 114-121
Doi:10.3906/biy-1204-20
View : 13 | Download : 3
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :Aerial parts of Daphne pontica were collected from Ilgaz-Çankırı, and D. oleoides subsp. oleoides was collected from 2 different localities (Ayrancı-Karaman and Ilgaz-Çankırı) in Turkey. The samples were subjected to hydrodistillation and microdistillation. The resulting volatile samples were analyzed both by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS), respectively. The main components for D. pontica were identified as hexahydrofarnesyl acetone (8.6%), carvacrol (8.5%), dihydroedulane II (4.7%), (E)-geranyl acetone (4.6%), and thymol (4.5%), while nonacosane (42.5% and 27.2%), hexadecanoic acid (24.4% and 20.0%), phytol (12.3%), and carvacrol (5.0%) were identified as the main components of D. oleoides subsp. oleoides obtained by hydrodistillation. Carvacrol (12.0%), thymol (7.7%), dihydroactinidiolide (7.2%), bicyclosesquiphellandrene (5.5%), and (Z)-3-hexenal (4.1%) were the major components in D. pontica, while carvacrol (27.2% and 25.4%), nonacosane (24.6%), (Z)-3-hexenal (18.5% and 2.5%), decane (7.4%), hexahydrofarnesyl acetone (7.4% and 2.2%), hexanal (6.6% and 1.5%), heptacosane (6.1%), nonanal (5.6% and 1.9%), thymol (5.1% and 2.3%), and phytol (5.0%) were identified in the D. oleoides subsp. oleoides isolated by microdistillation, respectively. In addition, the volatile components were evaluated for their ability to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals using a bioautographic thin layer chromatography (TLC) method, and the samples showed activity comparable with that of the tested standards, vitamins C and E.Keywords : Key words: Daphne sp., essential oil, volatiles, hydrodistillation, microdistillation, gas chromatography, gas chromatography-mass spectrometry, antioxidant activity