- Turkish Journal of Agriculture and Forestry
- Vol: 24 Issue: 1
- Effects of Genotype and Concentration of 2,4-D on Callus Induction and Plant Regeneration from Young...
Effects of Genotype and Concentration of 2,4-D on Callus Induction and Plant Regeneration from Young inflorescences of Dallisgrass ( Paspalum dilatatum Poir)
Authors : Ersin Can
Pages : 113-120
View : 6 | Download : 2
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :This study was conducted to determine the effects of genotype and 2,4-D concentrations on the callus induction and plant regeneration from young inflorescences of dallisgrass ( Paspalum dilatatumPoir). Segments of young inflorescences from six different ecotypes of dallisgrass were cultured on MS-medium containing different con-centrations of 2,4-D ( 2, 4, 6, 8 and 10 mg/l). The results of the study showed that the ecotypes were significantly different in cal-lus induction ratio, callus weight per petri dish and plant regeneration from the young inflorescences. With respect to the ecotypes, callus induction ratio varied from 17.5 % to 65 %, callus weight from 75.25 to 365.1 mg/petri dish and number of regenerates per inflorescence segment from 0.775 to 1.612. The callus induction ratio, callus weight and regeneration ratio were also signifi-cantly influenced by the 2,4-D concentrations. The segments cultured on the MS medium containing 6 mg/l of 2,4-D gave the high-est values of callus induction ratio (74 %), callus weight (369.3 mg/petri dish) and regeneration ratio (2.094 regenerates per seg-ment).Keywords : Turk. J. Agric. For., 24, (2000), 113-120. Full text: pdf Other articles published in the same issue: Turk. J. Agric. For., vol.24, iss.1.