- Turkish Journal of Agriculture and Forestry
- Vol: 37 Issue: 3
- Development of short gSSRs in G. arboreum and their utilization in phylogenetic studies
Development of short gSSRs in G. arboreum and their utilization in phylogenetic studies
Authors : Tayyaba Shaheen, Yusuf Zafar, James M. Stewart, Mehboob-ur Rahman
Pages : 288-299
Doi:10.3906/tar-1202-35
View : 8 | Download : 2
Publication Date : 9999-12-31
Article Type : Makaleler
Abstract :Microsatellite regions in DNA fragments of Gossypium arboreum were explored and PCR products 500-800 bp long were analyzed for genomic simple sequence repeats (gSSRs). From 39 segments, 23 short gSSRs were identified; on average they occurred every 1.6 kb. Primers were used to amplify fragments from 18 diploid species representing 6 diploid genomes and 1 tetraploid species, G. hirsutum (AD). The phylogeny of diploid genomes was subsequently determined. No primer amplified the DNA from all genotypes; however, amplification occurred in 59% of 437 PCR events. All genomes were grouped into 2 clusters (a and b). The obtained phylogeny of the species parallels previous reports. The strategy adopted here is an efficient method of identifying new gSSRs from species not extensively explored.Keywords : Key words: Gossypium, phylogenetic assessment, RAPD, short gSSRs