- The European Research Journal
- Vol: 9 Issue: 1
- Investigation of antioxidant and antimicrobial activities of walnut (Juglans regia L.) kernel septum
Investigation of antioxidant and antimicrobial activities of walnut (Juglans regia L.) kernel septum
Authors : Elif Azize Özşahin Delibaş, Esin Kiray
Pages : 87-96
Doi:10.18621/eurj.1056629
View : 25 | Download : 7
Publication Date : 2023-01-04
Article Type : Research
Abstract :Objectives: Walnut ( Juglans regia L.) kernel septum (or septa) (WKS), a traditional nutraceutical material in China, has not been explored in detail. In this study, antimicrobial activity, total phenolic content (TPC) and antioxidant-oxidant status of WKS was investigated in case it may be clinically important in the management of various complications. Methods: The WKS was extracted with ethanol in a Soxhlet device. TPC of WKS was analysed by using Folin-Ciocalteu’s method. Antioxidant activity was obtained by using Rel Assay Diagnostics kits. The antimicrobial activity of WKS was evaluated against two Gram-positive ( Staphylococcus aureus, Bacillus subtilis ), one Gram-negative bacteria ( Escherichia coli ) and one fungus ( Candida albicans ) strains using the agar diffusion method. Results: The TPC of WKS was found to be 119.42 ± 2.39 mg GAE/gDW. It was determined that total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) values were 7.542 ± 0.389 mmol/L, 3.718 ± 0.287 µmol/L and 0.049 ± 0.001, respectively. WKS selectively inhibited the growth of Gram-positive bacteria and fungus, while S. aureus was the most susceptible one with 16 mm of inhibition zone. Gram-negative bacteria was resistant to the extract. Conclusions: As far as we know, this paper is the first work that demonstrates the antioxidant-oxidant status of WKS by using the method described above, and moreover there are no scientific reports which have examined WKS in such a multidisciplinary experimental design. This study strongly supports the reported traditional use of WKS. Results indicated that WKS can be used as a pharmacological natural agent due to its high antioxidant and antimicrobial activities.Keywords : Juglans regia L, walnut kernel septum, antioxidant, total phenolic content, antimicrobial activity