- Selcuk Journal of Agriculture and Food Sciences
- Vol: 32 Issue: 1
- Morphological and Cytological Effects of In Vivo Colchicine Applications to 41B Rootstocks
Morphological and Cytological Effects of In Vivo Colchicine Applications to 41B Rootstocks
Authors : Zeki Kara, Osman Doğan, Kevser Yazar, Ali Sabir
Pages : 8-13
View : 11 | Download : 14
Publication Date : 2018-04-27
Article Type : Research Article
Abstract :Economically, vineyard has been cultivated in a very wide area and has become almost impossible to sustainable without grape rootstock because phylloxera pests have spread to globally. Since the existing suspended rootstocks cannot fully meet the requirements of the industry, rootstock breeding is also continuous. For this purpose, it is attempted to develop new rootstocks that are shorter in vegetation duration, more resistant to biotic and abiotic stress conditions. The development of tetraploid grape varieties as well as rootstocks in viticulture has attracted more interest in recent years. In this study, 41B rootstock was treated with colchicine applications (0.1%, 0.3%, 0.5%, 0.7%, 0.9% and 1.1%) at different times (24, 48, 72 and 96 hours) to induce ploidy at the beginning of the fast-growing period rooted single node cuttings and the effects of the treatments have been examined. Colchicine caused morphological changes according to dose and application time. While the number of stoma was decreased, stoma size, stoma width and stoma area were increased. On the other hand, no cytological changes were detected in flow cytometry (FC) assays. As a result, it has been understood that the shoots from 41B single nods cannot reach mitotic autopolyploid plants according to the FC analysis of a total of 240 materials from colchicine applications with different doses and application times. The exact frequency of colchicine-induced polyploidy induction in the 41B grape rootstock is less than 1 / 240th of that obtained from this study. However, due to the significant morphological differences detected in the colchicine treated material and limited variation in the FC analysis, the material is monitored by transferring to open are to follow the subsequent process.Keywords : Asma anacı, Poliploidi, Kimyasal mutasyon, Stoma, Flow sitometri