- Selcuk Journal of Agriculture and Food Sciences
- Vol: 18 Issue: 34
- Injecting The Prepubertal Laboratory Mice with A Mixture af Serine and Threonine and Its Effect on S...
Injecting The Prepubertal Laboratory Mice with A Mixture af Serine and Threonine and Its Effect on Spermatogenic Function
Authors : Hüseyin Baki Çiftçi
Pages : 41-45
View : 15 | Download : 18
Publication Date : 2004-12-25
Article Type : Research Article
Abstract :The aim of this study is to determine the effect of injecting a mixture of Serine and Threonine on spermatogenic function within testes of prepubertal mice (QS strain). Therefore, the mice were injected with Saline (Control) or a mixture of Serine and Threonine (Test) for 5 days. Following the last injection, the testes were removed and dehydrated then stained with PAS (Peri-odic Acid Schiff). The diameter of tubules, diameter of Leydig cells and the numbers of round spermatids were measured on sections of the testes. The number of stage round spermatids was decreased by Serine and Threonine injection (P<0.05). The total numbers of round spermatids per testis for control and test groups were detected as 1.660.32 and 1.060.05 (x106/mm3) respectively. This experiment shows that Serine and Threonine injection reduced the number of round spermatids while the diameters of Leydig cells and the diameters of tubules were not affected. The reason for reduced number of round spermatids might be a result of increased expression of TGF-s which might increase the apoptosis of the spermatogonia and this probably resulted with the reduction in the number of round spermatids.Keywords : Fare, serine, threonine, spermatogenik fonksiyon