- Selcuk Journal of Agriculture and Food Sciences
- Vol: 17 Issue: 32
- Boron Uptake of in Vitro Seedlings of Wheat (Triticum durum Desf.) and Barley (Hordeum vulgare L.) a...
Boron Uptake of in Vitro Seedlings of Wheat (Triticum durum Desf.) and Barley (Hordeum vulgare L.) as Determined by ICP-AES
Authors : Emine Atalay, Sait Gezgin, Mehmet Babaoğlu
Pages : 47-52
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Publication Date : 2003-12-25
Article Type : Research Article
Abstract :Boron (B) absorption of in vitro seedlings of wheat (Triticum durumDesf.) and barley (Hordeum vulgareL.) and its accumulation in various organs were investigated using an Inductively Coupled Plasma Atomic Emission Spectrometry (ICPAES). Dried plant samples (0.1-1 g) from 20-d old seedlings and plant culture media (5.0-6.0 g) were transferred into plastic bottles containing 10 ml of 65 % (v/v) nitric acid (HNO3) solution. Samples were ashed at 200°C for 40 min in a microwave (CEM-Mars x 5) followed by analyses in the ICP-AES device. The lowest concentration of B (2.2 μg B/g dry matter) accumulation was found in roots of Kiziltan-91 seedlings cultured in the control medium containing 0 mg/l B whereas the highest B accumulation (15.1 μg B/g dry matter) was detected again in roots of Kiziltan-91 in medium containing the highest concentration of B that was incorporated into the medium as H3BO3. Similarly, the highest level of B absorption by the shoots of Kiziltan-91 took place in media containing the highest level of boric acid (111.6 mg/l) with the lowest B concentration was found in shoots at the lowest medium B concentration (0 mg/l), showing that there was a linear correlation with respect to B content of the culture medium and B accumulation in the organs of Kiziltan-91. The B absorption response of barley seedlings were similar to that of wheat.Keywords : Bor alimi, MS besin ortami, arpa, bugday, ICP-AES